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KMID : 1161520120160050391
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Animal Cells and Systems
2012 Volume.16 No. 5 p.391 ~ p.399
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Hypoosmotic shock adaptation by prolactin involves upregulation of arginine vasotocin and osmotic stress transcription factor 1 mRNA in the cinnamon clownfish Amphiprion melanopus
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Park Mi-Seon
Kim Na-Na
Shin Hyun-Suk
Min Byung-Hwa
Kil Gyung-Suk
Cho Sung-Hwoan
Choi Cheol-Young
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Abstract
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We cloned cDNA-encoding arginine vasotocin (AVT) from the brain of the cinnamon clownfish Amphiprion melanopus, and that was predicted to encode a protein of 153 amino acids. We examined changes in the expression of AVT mRNA in the brain and arginine vasotocin receptor (AVTR) mRNA and osmotic stress transcription factor 1 (OSTF1) mRNA in the gills of the cinnamon clownfish using quantitative real-time PCR in an osmotically changing environment (seawater (35 psu) → brackish water (BW, 17.5 psu) and BW with prolactin [PRL]). The expression of AVT, AVTR, and OSTF1 mRNA in the brain and gills increased after transfer to BW, and the expression was repressed by PRL treatment. AVT-immunoreactive cells were almost consistently observed in the telencephalon. The plasma Na+ and Cl? levels decreased in BW, but the level of this parameter increased in BW with PRL treatment during salinity change. These results suggest that AVT, AVTR, and OSTF1 play important roles in hormonal regulation in osmoregulation organs, and that PRL improves the hyperosmoregulatory ability of cinnamon clownfish in BW environment.
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KEYWORD
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arginine vasotocin, arginine vasotocin receptor, cinnamon clownfish, osmotic stress transcription factor 1, prolactin
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